| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135164 |
| Chromosome: | chromosome 10 |
| Location: | 4900506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454700 | (1 of 1) IPR001611//IPR003591//IPR025875 - Leucine-rich repeat // Leucine-rich repeat, typical subtype // Leucine rich repeat 4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAACTCTTAACGTGGCATGGCTGAGAT |
| Internal bar code: | AGGCTGTATCGAGGCTGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 5.21562 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 3253 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTTGAGCACCTTGAGGC |
| Suggested primer 2: | CTGTTCGAGTGAGGAGGGAG |