| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135171 |
| Chromosome: | chromosome 5 |
| Location: | 2525047 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGCTGCACCGGGCGCTGATGCGGGTG |
| Internal bar code: | GATGATACAAGTGATAATGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 99.8701 |
| LEAP-Seq n confirming: | 2306 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGCCTGAGGTAAGTGAG |
| Suggested primer 2: | GTCCAGGTGGCTCTTGTGAG |