| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135172 |
| Chromosome: | chromosome 8 |
| Location: | 1844534 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGACTGCTTGCGAGAATCTGGAGGTCA |
| Internal bar code: | TCGGGGACGGCCAGAGCCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 94.8845 |
| LEAP-Seq n confirming: | 575 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAGGACAAGCACACTAA |
| Suggested primer 2: | GCAGGTGCTTGCTTGTATGA |