| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135180 |
| Chromosome: | chromosome 1 |
| Location: | 4551480 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031050 | (1 of 1) K15172 - transcription elongation factor SPT5 (SUPT5H, SPT5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGCCAGGAAGGTCATGTCTAGAATCTT |
| Internal bar code: | GATCGAGATAGGGACGTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 391 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTGCTCGTTGTTTCTCA |
| Suggested primer 2: | TGCGGGAAAACTACGTAACC |