| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135185 |
| Chromosome: | chromosome 7 |
| Location: | 1494378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323850 | PPP27 | (1 of 2) PTHR12210//PTHR12210:SF68 - NUCLEAR LIM INTERACTOR-INTERACTING FACTOR-RELATED // SUBFAMILY NOT NAMED; Phosphoprotein phosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGTAAAATGTGGCCTTACCTGCCAGTC |
| Internal bar code: | GGGACTACGTCGTCGCCGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 98.9174 |
| LEAP-Seq n confirming: | 3198 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACAAGGGAAGCACCATC |
| Suggested primer 2: | CAGGGACTCGCAAGAGGTAG |