| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135202 |
| Chromosome: | chromosome 14 |
| Location: | 688706 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612500 | (1 of 1) K05954 - protein farnesyltransferase subunit beta (FNTB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGCCTGCCAGCAAGTCAGCGATGCCT |
| Internal bar code: | GGCTCTTTCTTGGGTCAATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 94.9794 |
| LEAP-Seq n confirming: | 2762 |
| LEAP-Seq n nonconfirming: | 146 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAACGTCTCAGGTGGTGT |
| Suggested primer 2: | CCTCTGTGTGCTCCTTGACA |