| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135215 |
| Chromosome: | chromosome 6 |
| Location: | 6935017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296150 | (1 of 5) K15255 - ATP-dependent DNA helicase PIF1 [EC:3.6.4.12] (PIF1); PIF1-type helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCCGCCTCTCTTCCCCCTTGCCCCAGG |
| Internal bar code: | ATGCACTGGTGACGTGTGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 97.7226 |
| LEAP-Seq n confirming: | 472 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTCGGTGAGGGTGGCTA |
| Suggested primer 2: | TGAGCTACCGGAATACCCAC |