| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135219 |
| Chromosome: | chromosome 1 |
| Location: | 5554748 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039450 | (1 of 2) PTHR34548//PTHR34548:SF2 - FAMILY NOT NAMED // PROTEIN TIC 21, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGCGCGCGGCGCGCACCATGCGGAAGA |
| Internal bar code: | CCAACGGCTTAATAGTTGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1411 |
| LEAP-Seq percent confirming: | 3.57143 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCATGACCTTGTGGTT |
| Suggested primer 2: | ACCACCTGTAAAGCAATGCC |