| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135219 |
| Chromosome: | chromosome 1 |
| Location: | 5554776 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039450 | (1 of 2) PTHR34548//PTHR34548:SF2 - FAMILY NOT NAMED // PROTEIN TIC 21, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGGCATGGCGCTCAAGTCCGTGGACGG |
| Internal bar code: | AACTTAGATCCGGCTGAATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 98.6445 |
| LEAP-Seq n confirming: | 2547 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCATGACCTTGTGGTT |
| Suggested primer 2: | ACCACCTGTAAAGCAATGCC |