Insertion junction: LMJ.RY0402.135220_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.135220
Chromosome:chromosome 7
Location:1185819
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g320950 (1 of 1) PF09465 - Lamin-B receptor of TUDOR domain (LBR_tudor) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGCATGCGCATTTCCATGGCCACTCCC
Internal bar code:ACAGTCGCATTATGTATTATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:568
LEAP-Seq percent confirming:76.5292
LEAP-Seq n confirming:1076
LEAP-Seq n nonconfirming:330
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTGCGTTGACGCTGATTA
Suggested primer 2:TTGGACGCCCTAATGAGTTC