| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135224 |
| Chromosome: | chromosome 9 |
| Location: | 7498519 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413950 | EFH6 | EF-hand Calcium binding protein; (1 of 5) PTHR23050:SF90 - CENTRIN-3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCCTGAAATGCCCCCGCCCCAAGCTGG |
| Internal bar code: | GCCGTATCAAGCTGGGTCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 288 |
| LEAP-Seq percent confirming: | 95.5121 |
| LEAP-Seq n confirming: | 1809 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCATACAACACGCACGAC |
| Suggested primer 2: | AGCCTGCCACAGTCTTGAGT |