Insertion junction: LMJ.RY0402.135231_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.135231
Chromosome:chromosome 9
Location:1717263
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396750 CSE18 Predicted protein of CSE family; (1 of 1) IPR000601//IPR022409 - PKD domain // PKD/Chitinase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGAACAACAGTGTGCCAAGAGGCAAGCA
Internal bar code:TACGATGAGCTGGTACGACACA

Confirmation - LEAP-Seq

LEAP-Seq distance:734
LEAP-Seq percent confirming:98.2823
LEAP-Seq n confirming:1316
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGTGGAGGGGGAGGAGAG
Suggested primer 2:CTTTCCTCACCCCTCATTCA