| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135235 |
| Chromosome: | chromosome 3 |
| Location: | 3607813 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168550 | AOT3 | Amino acid transporter; (1 of 1) K15015 - solute carrier family 32 (vesicular inhibitory amino acid transporter) (SLC32A, VGAT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAACGTGCTGTTGCCGCCCGAGCTCTGG |
| Internal bar code: | AGGCGCCTTGGACACGTTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.4813 |
| LEAP-Seq n confirming: | 959 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCACTGAAACCTGAGCC |
| Suggested primer 2: | CCACCCACTCACTCACCTTT |