| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135237 |
| Chromosome: | chromosome 2 |
| Location: | 4156372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098150 | XYK1 | (1 of 1) 2.7.1.17 - Xylulokinase / Xylulose kinase; Xylulose kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCAGCATGTAGCACAGCATCAGCGGCC |
| Internal bar code: | TTCGCAGAAAACTCTACACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 98.3113 |
| LEAP-Seq n confirming: | 1863 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTTCCCTGTAAGCAAT |
| Suggested primer 2: | CTGTGTGGTGCAGTAGGCAT |