| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135271 |
| Chromosome: | chromosome 4 |
| Location: | 1613232 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213150 | MAE13 | MATE efflux family protein; (1 of 5) PTHR11206//PTHR11206:SF94 - MULTIDRUG RESISTANCE PROTEIN // DNA-DAMAGE-INDUCIBLE PROTEIN F | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGGCGGTGCAGGTGGGCGAGCGTGTG |
| Internal bar code: | GGATGCGGTTTGTTTATTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 80 |
| LEAP-Seq percent confirming: | 99.8631 |
| LEAP-Seq n confirming: | 2188 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTACAACTTCGCTTCCT |
| Suggested primer 2: | GAAGTAGTTTCGGCTGACGC |