Insertion junction: LMJ.RY0402.135288_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.135288
Chromosome:chromosome 16
Location:1018639
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g649150 (1 of 1) PF13414//PF13920 - TPR repeat (TPR_11) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGATCATTCATGAGTAAGATTGGCTCG
Internal bar code:CGAGGTGCTAACCGCTCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:306
LEAP-Seq percent confirming:99.5334
LEAP-Seq n confirming:640
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTGTCATGACCAGTTGC
Suggested primer 2:CCTGTACCACGTCCCTGACT