| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135298 |
| Chromosome: | chromosome 8 |
| Location: | 371951 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358572 | (1 of 5) PF03094 - Mlo family (Mlo) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATAAATGGCACTTCCTGCTTATCCTCTC |
| Internal bar code: | AGGCAGAGTGGATCAGGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 97.9957 |
| LEAP-Seq n confirming: | 1369 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCTGACTGAACCAGTTG |
| Suggested primer 2: | ATTCGGTGTAGTTTGGGCTG |