Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.135305 |
Chromosome: | chromosome_7 |
Location: | 2658967 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g330650 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AATACATGGCCAAGATTCTGGGTACCATCT |
Internal bar code: | CACGGAGGGTGGTGCCAAGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 800 |
LEAP-Seq percent confirming: | 99.3585 |
LEAP-Seq n confirming: | 2478 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACACTTATCGAGGGTGGT |
Suggested primer 2: | CGGCGGAGTAATTGATGATT |