| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135342 |
| Chromosome: | chromosome 6 |
| Location: | 3097962 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274950 | (1 of 1) K18953 - factor associated with neutral sphingomyelinase activation (NSMAF, FAN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGTGATACGGTAGTCAGCTTCGTGCT |
| Internal bar code: | TAGGGTGCTACTCGGTCGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 99.6829 |
| LEAP-Seq n confirming: | 7231 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTAGCCGAAGATGAGG |
| Suggested primer 2: | GTGGACTCCATGCCTCAACT |