| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135349 |
| Chromosome: | chromosome 3 |
| Location: | 5393139 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184700 | (1 of 1) PTHR31810//PTHR31810:SF6 - FAMILY NOT NAMED // METACASPASE-4-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGTCTTGTGGGGGGCGGGCGTGGTTCA |
| Internal bar code: | CCCCACGACGTAGAACCGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 97.9498 |
| LEAP-Seq n confirming: | 5303 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGACCCTTGCTTTCGCAC |
| Suggested primer 2: | AGACATGTCACTGCGCTCAC |