| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135350 |
| Chromosome: | chromosome_6 |
| Location: | 6335309 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g291900 | PHD1 | Phosducin-like protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCACTGGCCCTGCTGTGATTAAGAGAGGTT |
| Internal bar code: | AAGCAGTGGGGCCGACAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1330 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAGCAGCGGTATTGAAG |
| Suggested primer 2: | TCCTCGTCCACGTAATCCTC |