Insertion junction: LMJ.RY0402.135353_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.135353
Chromosome:chromosome 16
Location:1525084
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g653300 BLZ19 bZIP transcription factor; (1 of 21) IPR004827 - Basic-leucine zipper domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACAATGAACAACCGCAAGGTTTGACTTC
Internal bar code:CGTCGCGGAGTCAAATGCGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:794
LEAP-Seq percent confirming:99.4413
LEAP-Seq n confirming:2314
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CATTTCCAGGGAAGAGGACA
Suggested primer 2:TGCAGGAGACGCTTAGGATT