| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135355 |
| Chromosome: | chromosome 14 |
| Location: | 3375471 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630200 | FAP312 | Flagellar Associated Protein 312 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGTTGCCTTGGGCTCGCGATTGTTTCT |
| Internal bar code: | AGTTAGTCGTGGCCGTGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 15.0771 |
| LEAP-Seq n confirming: | 313 |
| LEAP-Seq n nonconfirming: | 1763 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCACATCCTTGCAACAC |
| Suggested primer 2: | CACGAGTGAGAGAGCGAGTG |