| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135355 |
| Chromosome: | chromosome 14 |
| Location: | 3375484 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630200 | FAP312 | Flagellar Associated Protein 312 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTTGCAGGGCTTTGTGATTATCCTCTC |
| Internal bar code: | CGGAGGTGAGACTTGCTTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGTACCGCTTTTCCAT |
| Suggested primer 2: | CACGAGTGAGAGAGCGAGTG |