| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135374 |
| Chromosome: | chromosome 2 |
| Location: | 6736014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118300 | RHE,HEL12 | (1 of 1) PTHR24031:SF330 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 7; DEAD box ATP-dependent RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCTCTCAAACCTGCCCTCAGACACCTG |
| Internal bar code: | AAGCAACTCCGGAACGTCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 99.7024 |
| LEAP-Seq n confirming: | 3685 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGCGCTATGGTTTTGTG |
| Suggested primer 2: | CTGGTGTTCTCCAGGTTGGT |