Insertion junction: LMJ.RY0402.135426_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.135426
Chromosome:chromosome 5
Location:739526
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g248650 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAGGCAAAGGAGGAGTTGTTGGCAGCCC
Internal bar code:GCTAGGTGCCTTGCGGTATCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGGTTTTAGGGGAAGTG
Suggested primer 2:GAAATGCAGCACATAGCGAA