Insertion junction: LMJ.RY0402.135442_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.135442
Chromosome:chromosome 3
Location:8997261
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g209729 (1 of 8) IPR015915 - Kelch-type beta propeller CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAGTGCGCTGTTCAGAGACCTCCAGCAC
Internal bar code:GTTGAACGCCCAACCCGGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:99.6776
LEAP-Seq n confirming:4946
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TACTCTCAATGCATGCAGCC
Suggested primer 2:ACACAGGAGCCAGGAGAGAA