| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135455 |
| Chromosome: | chromosome_17 |
| Location: | 3439376 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g724550 | DIP13 | Deflagellation inducible protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGGACTTGACCGCTACATGGATACATCAA |
| Internal bar code: | TGACCGTGGTCTTTTGCGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 99.6696 |
| LEAP-Seq n confirming: | 5129 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCGGGTATTGACTGTGT |
| Suggested primer 2: | GCTCTGCAGAACCACAACAA |