| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135466 |
| Chromosome: | chromosome 7 |
| Location: | 2135689 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTATTTCCTTCATGCTTTATGTCTACC |
| Internal bar code: | TACCGACAAGAGAGGGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1062 |
| LEAP-Seq percent confirming: | 99.5667 |
| LEAP-Seq n confirming: | 6894 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTACATTCATGGCAACTG |
| Suggested primer 2: | CATCACAAACTTGACCGTGC |