Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.135483 |
Chromosome: | chromosome 12 |
Location: | 8567025 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g547351 | (1 of 5) IPR001054//IPR006189//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // CHASE domain // Nucleotide cyclase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAGTCCCCATGGCTTTCTCTTGCTCGT |
Internal bar code: | GCCCTTTGTAAGATGGCAGCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 775 |
LEAP-Seq percent confirming: | 74.7567 |
LEAP-Seq n confirming: | 1229 |
LEAP-Seq n nonconfirming: | 415 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTAGTACGCCTGCCTTCCA |
Suggested primer 2: | CGGAGAGGTGTAGGAGTGGA |