| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135492 |
| Chromosome: | chromosome 12 |
| Location: | 7556847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555550 | (1 of 1) K05768 - gelsolin (GSN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGAGTGGTCACGGCCTTAGCTGACACGC |
| Internal bar code: | CCGAAGTCCGTGATTCAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 98.6507 |
| LEAP-Seq n confirming: | 1974 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATAGACCCCCAGCACCT |
| Suggested primer 2: | CTCTCTGAGGGTCGGTTCTG |