Insertion junction: LMJ.RY0402.135505_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.135505
Chromosome:chromosome_2
Location:6518160
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g116650 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GAGGAACAGAATGCTGGCGTACGACCAGGC
Internal bar code:CTGGGCGTTCTGCGCAACATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:159
LEAP-Seq percent confirming:98.26
LEAP-Seq n confirming:960
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGTGGTCAGCTTTCGAT
Suggested primer 2:CGTGTAGGGGATGCTTTGTT