| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135506 |
| Chromosome: | chromosome 2 |
| Location: | 1866297 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087450 | (1 of 4) PTHR16255:SF4 - SPORULATION PROTEIN RMD8 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCGCCTGATGGGCGAGGAGTGGGGCT |
| Internal bar code: | GGTCCCGTTGTTTACTAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 99.5236 |
| LEAP-Seq n confirming: | 2298 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGCAGCGGTAGTTTGA |
| Suggested primer 2: | CCAGGTCTCAAGCAGAAAGG |