| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135518 |
| Chromosome: | chromosome 10 |
| Location: | 2821420 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439450 | (1 of 7) PF05517 - p25-alpha (p25-alpha) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAGATCGCTCGCGGGCCGCTCGCTCGA |
| Internal bar code: | GAGAAACATTATGGTCGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 99.6957 |
| LEAP-Seq n confirming: | 983 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGGTCCAATGATAAGT |
| Suggested primer 2: | GAGATGTCGCCTGCACAGTA |