| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135523 |
| Chromosome: | chromosome 14 |
| Location: | 1297166 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616700 | (1 of 2) 2.6.1.57 - Aromatic-amino-acid transaminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAAACGCGACCAACGCCGGGCTGCCCA |
| Internal bar code: | TAATAATTCTCAAACTTCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 95.6811 |
| LEAP-Seq n confirming: | 288 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACCGAGTCCAAGGTACG |
| Suggested primer 2: | CGACTCCTATGACCCCTCAA |