Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.135524 |
Chromosome: | chromosome_14 |
Location: | 1578537 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g618750 | FAP246 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAGGAGTAGATGAACGCATCCGGCGGCACA |
Internal bar code: | GTCGCCGAGTAGCCATTAAAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 148 |
LEAP-Seq percent confirming: | 99.5851 |
LEAP-Seq n confirming: | 240 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACGTGGTCAGTCTGGCT |
Suggested primer 2: | TGCATTGAGCGAGTCAAAAC |