| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135555 |
| Chromosome: | chromosome 7 |
| Location: | 5449501 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350500 | TSV,TSV1 | Valyl-tRNA synthetase; (1 of 1) PTHR11946//PTHR11946:SF76 - ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES // ATP BINDING/VALINE-TRNA LIGASE/AMINOACYL-TRNA LIGASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGACCAGCAGAGCACCACGAGCCAACC |
| Internal bar code: | GATTTCACCGTGTTACGCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.2126 |
| LEAP-Seq n confirming: | 252 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGAAGCTGGAGTCCTGA |
| Suggested primer 2: | TGCAGCATCTTCTCCACAAC |