| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135571 |
| Chromosome: | chromosome 4 |
| Location: | 1285091 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214850 | (1 of 1) IPR000104//IPR000253 - Antifreeze protein, type I // Forkhead-associated (FHA) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCGGGGCAATGTGGCATGCCGGGATT |
| Internal bar code: | AACCGATTCATCCCTTTACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 96.8421 |
| LEAP-Seq n confirming: | 276 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAGGAAGAGGAGGAG |
| Suggested primer 2: | CGTAGCCTGTAGCGATTTCC |