| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135597 |
| Chromosome: | chromosome_16 |
| Location: | 1169531 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g650550 | FAP103 | Flagellar Associated Protein, nucleoside diphosphate kinase-like | antisense | 3'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTCAACACATTGCTTCGGCGTGCGGAGAC |
| Internal bar code: | GTGGAGGGGAGTCCGTCGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 858 |
| LEAP-Seq percent confirming: | 99.8964 |
| LEAP-Seq n confirming: | 1929 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTGTGCAACTATCAGGA |
| Suggested primer 2: | TGGTTACGAAAGATGGCTCC |