| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135598 |
| Chromosome: | chromosome 10 |
| Location: | 31558 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417650 | (1 of 1) 2.1.1.207 - tRNA (cytidine(34)-2'-O)-methyltransferase / tRNA methyltransferase L | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTAAAACTCCTTTGGTTTGATGGTTGCC |
| Internal bar code: | GGTTGCGGCCCGTCATTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 99.6094 |
| LEAP-Seq n confirming: | 3570 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCCTTCATTCGTCATTT |
| Suggested primer 2: | GGTATCTCGTTCAGGCCGTA |