| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135618 |
| Chromosome: | chromosome 4 |
| Location: | 1783070 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212050 | (1 of 2) IPR000104//IPR000253//IPR008984 - Antifreeze protein, type I // Forkhead-associated (FHA) domain // SMAD/FHA domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAGAGGCCTTGCATAGAACTGCAGTGT |
| Internal bar code: | CGACTGGAGTAGTGAGCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 99.7777 |
| LEAP-Seq n confirming: | 3142 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCACTGTGTGTAGACCT |
| Suggested primer 2: | TACCAGCAGTCGTACCAGCA |