| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135645 |
| Chromosome: | chromosome 6 |
| Location: | 5831902 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287750 | PYR8 | Dihydroorotase dehydrogenase; (1 of 1) 1.3.1.14 - Dihydroorotate dehydrogenase (NAD(+)) / Orotate reductase (NADH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACCGGCACGCAGTAGCCTGCCTGCCG |
| Internal bar code: | ATCGTTGATTAGGTAAGATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 29.3998 |
| LEAP-Seq n confirming: | 289 |
| LEAP-Seq n nonconfirming: | 694 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGGACGAGGAGAGAGG |
| Suggested primer 2: | TTTGGGATATCATGGACCGT |