| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135682 |
| Chromosome: | chromosome 8 |
| Location: | 1149464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362750 | (1 of 1) K01173 - endonuclease G, mitochondrial (ENDOG) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATGTGCGCTGTGTTTTTTTACTGCGGT |
| Internal bar code: | ATACGATACGCTAATCCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 96.1527 |
| LEAP-Seq n confirming: | 3224 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGCCTCGTCAACATCAG |
| Suggested primer 2: | GCAGATGGAGGATAAGAGCG |