| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135695 |
| Chromosome: | chromosome 4 |
| Location: | 1565603 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213450 | VPS2A | Subunit of the ESCRT-III complex; (1 of 1) K12191 - charged multivesicular body protein 2A (CHMP2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCCTACCCTGCCCCATGGACCTGCCC |
| Internal bar code: | ATATGACTAGGATGGGAAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 89.1706 |
| LEAP-Seq n confirming: | 2849 |
| LEAP-Seq n nonconfirming: | 346 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTAGGCCGCAAACAAGATG |
| Suggested primer 2: | AGACGGACAACCTGGTGAAC |