| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135712 |
| Chromosome: | chromosome 3 |
| Location: | 8312734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199050 | (1 of 1) IPR000595//IPR000719//IPR000961//IPR002290//IPR011009//IPR014710//IPR018490//IPR020635//IPR027916 - Cyclic nucleotide-binding domain // Protein kinase domain // AGC-kinase, C-terminal // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // RmlC-like jelly roll fold // Cyclic nucleotide-binding-like // Tyrosine-protein kinase, catalytic domain // Protein kinase-like domain, Apicomplexa | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATACACAGGACGGCGCAGGGCGGGTTGC |
| Internal bar code: | GAACTCATCTCCGATTGCCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 99.6606 |
| LEAP-Seq n confirming: | 4111 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTGTGTGTGTGCGTGTG |
| Suggested primer 2: | TAACAGGTCTTGAGCGCCTT |