Insertion junction: LMJ.RY0402.135728_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.135728
Chromosome:chromosome 12
Location:7177207
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g558700 FAP67 Flagellar Associated Protein 67; (1 of 4) K00940 - nucleoside-diphosphate kinase (ndk, NME) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTGGACATCAAGAACCGCCGGCACTTC
Internal bar code:CGTCATGTCGTAGTCAGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:99.7799
LEAP-Seq n confirming:2267
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGCGATTTCAAGCACAGA
Suggested primer 2:CAGAGCATTTGGCACTTGAA