Insertion junction: LMJ.RY0402.135756_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.135756
Chromosome:chromosome 17
Location:6125001
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g741500 KIL7 Putative kinesin-like motor protein; (1 of 1) 3.4.21.72//3.6.4.4 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease // Plus-end-directed kinesin ATPase / Kinesin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGGAGCTGCTGCCACAGCAGCACCCAG
Internal bar code:AAGTAGTCGGTCCCGGGTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:84.4898
LEAP-Seq n confirming:207
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGGAGAAGCAGCTCAAG
Suggested primer 2:TTCTGGCAAAGTTTCGTCCT