| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135759 |
| Chromosome: | chromosome 13 |
| Location: | 1987476 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576650 | HIS4 | Bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase; (1 of 1) 3.6.1.31 - Phosphoribosyl-ATP diphosphatase / Phosphoribosyladenosine triphosphate pyrophosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAAGCCCTGCACAAGCAGCGACAGCAG |
| Internal bar code: | CGGCGGGACGCCTTGGTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 99.6074 |
| LEAP-Seq n confirming: | 3298 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTGCGTTAGGATTTGGT |
| Suggested primer 2: | CTCGATCCCTCCCTAGATCC |