Insertion junction: LMJ.RY0402.135770_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.135770
Chromosome:chromosome 7
Location:4109548
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g342920 (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCGCAGAGGTCCCGTACCCACAGGGCGT
Internal bar code:GTGCCCTACCCTGTACGGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:725
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTGAATCGAACCCAACG
Suggested primer 2:ACGCCCTTACTGTTTCATGG