| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135785 |
| Chromosome: | chromosome 3 |
| Location: | 3545767 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167887 | (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTGGATGGAGTTGGCTTCGCTCATTA |
| Internal bar code: | TGTAATATCTATTCATTGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCCTGGCATGTTTACC |
| Suggested primer 2: | ATTGTGCTACACCGTGCTTG |